Part:BBa_K524004:Design
pir gene
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 438
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Homologous sites in primers designed by researchers to clone the pir gene into the E. coli BW25141 genome were used to clone out and standardize the pir gene. This is to avoid truncation of pir gene, and therefore junk sequences of 154 base pairs can be found downstream of the pir CDS.
Source
Cloned out and standardized from the genome of strain BW25141 (courtesy of The Coli Genetic Stock Center).
References
F Wu, I Goldberg, and M Filutowicz.(1992).Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K, Nucleic Acids Res. 1992 February 25; 20(4): 811–817.
F Wu, I Goldberg, and M Filutowicz.(1994).Binding of DnaA protein to a replication enhancer counteracts the inhibition of plasmid R6K gamma origin replication mediated by elevated levels of R6K pi protein, J Bacteriol. 1994 November; 176(22): 6795–6801.
M Filutowicz, G Davis, A Greener, and D R Helinski.(1985).Autorepressor properties of the pi-initiation protein encoded by plasmid R6K, Nucleic Acids Res. 1985 January 11; 13(1): 103–114.
M Filutowicz, M J McEachern, and D R Helinski.(1986).Positive and negative roles of an initiator protein at an origin of replication, Proc Natl Acad Sci U S A. 1986 December; 83(24): 9645–9649.